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Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
Published on: December 1, 2020
Biophysical methods in drug discovery from small molecule to pharmaceutical
Geoffrey Holdgate1, Stefan Geschwindner, Alex Breeze
1AstraZeneca R&D, Macclesfield, UK.
Methods in Molecular Biology (Clifton, N.J.)
|June 5, 2013
Summary
Biophysical methods are essential for drug discovery, enabling the characterization of molecular interactions. High-throughput instruments now allow broader application of these techniques for understanding binding kinetics and thermodynamics.
Area of Science:
- Drug discovery and development
- Biophysics
- Molecular interactions
Background:
- Biophysical methods were once limited to specialized labs but are now widely adopted.
- Automated, high-throughput instruments have increased accessibility.
- These methods are crucial for understanding molecular recognition.
Purpose of the Study:
- To provide an overview of the drug discovery process.
- To explain how biophysical methods aid drug discovery.
- To highlight specific techniques and their applications.
Main Methods:
- Surface Plasmon Resonance (SPR)
- Isothermal Titration Calorimetry (ITC)
- Nuclear Magnetic Resonance (NMR)
- Mass Spectrometry (MS)
- Thermal unfolding
Main Results:
- Biophysical methods can measure equilibrium binding constants.
- They characterize kinetic and thermodynamic components of binding.
- Stoichiometry, binding mode, and conformational changes can be determined.
- These techniques provide a comprehensive understanding of binding processes.
Conclusions:
- Biophysical methods offer a powerful toolbox for drug discovery.
- Techniques like SPR and ITC are vital for characterizing molecular interactions.
- Application of these methods influences project progression and outcomes.
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