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Related Experiment Videos

Jacalin: a lectin mitogenic for human CD4 T lymphocytes.

N Pineau1, P Aucouturier, J C Brugier

  • 1Laboratory of Immunology and Immunopathology (CNRS URA 1172), Poitiers University Hospital, France.

Clinical and Experimental Immunology
|June 1, 1990
PubMed
Summary

Purified jacalin, a jackfruit lectin, stimulates human T lymphocytes, specifically CD4+ T cells, in a monocyte-dependent manner. This lectin

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Area of Science:

  • Immunology
  • Biochemistry

Background:

  • Jackfruit seeds contain jacalin, a major protein and lectin.
  • Crude jackfruit extracts stimulate lymphocytes, but purified jacalin's mitogenic effects are not well-understood.

Purpose of the Study:

  • To investigate the mitogenic properties of purified jacalin on human peripheral blood lymphocytes.
  • To elucidate the specific lymphocyte subsets and mechanisms involved in jacalin-induced proliferation.

Main Methods:

  • Studied the proliferative response of human peripheral blood cells to purified jacalin.
  • Utilized phenotypic analysis and studies of isolated T cell subpopulations.
  • Investigated monocyte dependence and antibody inhibition.

Main Results:

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  • Purified jacalin is mitogenic for human lymphocytes via its lectin-binding site, inhibited by IgA.
  • Jacalin specifically induces proliferation of CD4+ T cells, not CD8+ T cells or B cells.
  • The response is dependent on autologous monocytes and shows distinct kinetics compared to phytohemagglutinin.

    • Conclusions:

    • Jacalin acts as a T cell mitogen, primarily targeting CD4+ T lymphocytes.
    • The mechanism involves lectin binding and requires monocyte co-stimulation.
    • Further research is needed to understand the restricted CD4+ T cell responsiveness.