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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Mass Spectrometry: Molecular Fragmentation Overview01:20

Mass Spectrometry: Molecular Fragmentation Overview

The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can occur at...
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...

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Related Experiment Video

Updated: May 10, 2026

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
10:59

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

DNA fragmentation by gamma radiation and electron beams using atomic force microscopy.

Luis Nieto González1, João D T Arruda-Neto, Monica A Cotta

  • 1Departamento de Ciência e Tecnologia, Universidade Estadual de Santa Cruz, Ilhéus, BA Brazil.

Journal of Biological Physics
|June 5, 2013
PubMed
Summary

Atomic force microscopy (AFM) revealed distinct DNA fragment patterns after gamma irradiation, contrasting with continuous shattering from electron beams. AFM offers high-resolution insights into radiation-induced DNA damage.

Keywords:
AFMElectron beamsFragment-size distributionsGamma radiationPlasmid DNA

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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
08:30

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

Area of Science:

  • Biophysics
  • Materials Science
  • Radiation Biology

Background:

  • Understanding DNA damage is crucial for radiation therapy and safety.
  • Traditional methods for analyzing DNA damage have limitations in resolution and scope.
  • Atomic Force Microscopy (AFM) offers potential for high-resolution imaging of biological molecules.

Purpose of the Study:

  • To investigate and compare DNA fragment size distributions after irradiation with gamma rays and electron beams.
  • To evaluate the utility of Atomic Force Microscopy (AFM) for high-resolution analysis of radiation-induced DNA damage.
  • To explore the influence of irradiation type and dose rate on DNA fragmentation patterns.

Main Methods:

  • Irradiation of double-stranded pBS plasmid DNA with gamma rays (1-12 kGy) and electron beams (1-10 kGy).
  • Direct visualization and fragment-size distribution analysis using Atomic Force Microscopy (AFM) in non-tapping mode.
  • Application of an improved AFM methodology for enhanced nanometer-resolution imaging.

Main Results:

  • Gamma irradiation resulted in discrete-like DNA fragment patterns, suggesting modulation by plasmid base pair composition.
  • Electron beam irradiation, even at high dose rates, produced continuous distributions of highly fragmented DNA.
  • AFM provided detailed, high-resolution data on DNA fragmentation, complementing existing techniques.

Conclusions:

  • AFM is a valuable tool for high-resolution measurement of radiation damage to DNA.
  • Different radiation types (gamma vs. electron beams) induce distinct DNA fragmentation patterns.
  • AFM can provide novel information on DNA damage mechanisms and characteristics.