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Gradients in surface nanotopography used to study platelet adhesion and activation
M Hulander1, A Lundgren, L Faxälv
1Department of Chemistry and Molecular Biology, University of Gothenburg, Medicinaregatan 9E, 413 90 Gothenburg, Sweden. mats.hulander@cmb.gu.se
Colloids and Surfaces. B, Biointerfaces
|June 5, 2013
Summary
Platelet adhesion and activation on nanostructured gold surfaces depend on nanoparticle coverage. Peak adhesion occurred at 23% coverage, while activation was highest on smooth surfaces, suggesting nano-topography influences cell behavior.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Cell Biology
Background:
- Nanostructured surfaces are crucial for biomaterial design.
- Understanding cell interactions with nanotopography is key for blood-contacting devices.
Purpose of the Study:
- To investigate the effect of surface nanotopography gradients on human platelet adhesion and activation.
- To determine the relationship between nanoparticle coverage and platelet response.
Main Methods:
- Preparation of gold surfaces with nanotopography gradients using gold nanoparticles.
- Adsorption of human fibrinogen.
- Analysis of platelet adhesion and activation on surfaces with varying nanoparticle coverage.
Main Results:
- A peak in platelet adhesion was observed at 23% nanoparticle surface coverage.
- Platelet activation was highest on smooth surfaces and inversely correlated with nanoparticle coverage.
- A lower limit for sensing nano-roughness by platelets was suggested for 36nm particles.
Conclusions:
- Surface nanotopography, including nanoparticle size and inter-particle distance, significantly influences platelet response more than wettability.
- Reduced platelet activation on nanostructured surfaces and a cut-off in activation suggest potential for designing advanced blood-contacting biomaterials.
