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Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

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Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
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Interactions among rice ORC subunits.

Deyong Tan1, Qundan Lv, Xinai Chen

  • 1The State Key Laboratory of Plant Physiology and Biochemistry; College of Life Sciences; Zhejiang University; Hangzhou, PR China.

Plant Signaling & Behavior
|June 5, 2013
PubMed
Summary

Researchers identified six origin recognition complex (ORC) subunits in rice, crucial for DNA replication. Key subunit interactions suggest conserved functions in eukaryotes, with OsORC1 and OsORC6 showing no interactions.

Keywords:
interactionorigin recognition complex (ORC)ricesubunittemperature

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The origin recognition complex (ORC) is essential for DNA replication initiation in eukaryotes.
  • Understanding ORC subunit composition and interactions is key to deciphering replication control.

Purpose of the Study:

  • To identify and characterize the six subunits of the rice ORC (Oryza sativa ORC).
  • To investigate the interactions between rice ORC subunits and their conservation.

Main Methods:

  • Experimental isolation and sequencing of rice ORC subunits.
  • Reverse transcription polymerase chain reaction (RT-PCR) to analyze gene expression.
  • Yeast two-hybrid assays to determine protein-protein interactions.

Main Results:

  • All six expected ORC subunits were identified in rice (OsORC1-6).
  • Rice ORC gene expression showed no significant difference between 26°C and 34°C.
  • Yeast two-hybrid assays revealed interactions between OsORC2, -3, and -5, forming a core complex with OsORC4, suggesting conserved eukaryotic ORC assembly.

Conclusions:

  • Rice possesses a complete six-subunit ORC complex.
  • The core interactions of the ORC complex appear conserved across eukaryotic evolution.
  • OsORC1 and OsORC6 do not interact with the identified core complex subunits.