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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: May 10, 2026

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
08:43

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

Stochastic optical reconstruction microscopy (STORM): a method for superresolution fluorescence imaging.

Mark Bates, Sara A Jones, Xiaowei Zhuang

    Cold Spring Harbor Protocols
    |June 5, 2013
    PubMed
    Summary
    This summary is machine-generated.

    Stochastic optical reconstruction microscopy (STORM) overcomes optical limits by localizing single fluorescent molecules. This superresolution technique significantly enhances imaging resolution for biological ultrastructure.

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    Test Samples for Optimizing STORM Super-Resolution Microscopy
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    Published on: September 6, 2013

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    Last Updated: May 10, 2026

    Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
    08:43

    Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

    Published on: June 24, 2017

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    Test Samples for Optimizing STORM Super-Resolution Microscopy
    16:52

    Test Samples for Optimizing STORM Super-Resolution Microscopy

    Published on: September 6, 2013

    Area of Science:

    • Biophysics
    • Optical Microscopy
    • Cell Biology

    Background:

    • Conventional optical microscopy has limited spatial resolution (∼0.2 µm), hindering observation of nanoscale biological structures.
    • Subcellular components and molecular complexes crucial for biological function operate at nanometer to micrometer scales.

    Purpose of the Study:

    • To introduce Stochastic Optical Reconstruction Microscopy (STORM) as a superresolution imaging method.
    • To detail the principles, instrumentation, and applications of STORM for biological imaging.

    Main Methods:

    • Utilizes optically switchable fluorophores that cycle between fluorescent and non-fluorescent states.
    • Employs high-accuracy localization of individual fluorophores to reconstruct super-resolution images.
    • Covers STORM microscope design, imaging procedures, data analysis, and multicolor/3D STORM.

    Main Results:

    • Demonstrates a resolution improvement of an order of magnitude over conventional optical microscopy.
    • Achieves theoretically unlimited spatial resolution, surpassing traditional light microscopy limitations.
    • Successfully imaged cultured cells with enhanced detail.

    Conclusions:

    • STORM provides a powerful, generally applicable method for high-resolution biological imaging.
    • The technique requires relatively simple experimental apparatus.
    • Offers a significant advancement for visualizing subcellular structures and molecular complexes.