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Updated: May 10, 2026

The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression
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In-depth tanscriptomic analysis on giant freshwater prawns.

Maizatul Izzah Mohd-Shamsudin1, Yi Kang, Zhao Lili

  • 1Genomics and Evolutionary Biology Lab, Centre for Research in Biotechnology for Agriculture (CEBAR) and Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.

Plos One
|June 5, 2013
PubMed
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This study generated extensive transcriptome data for the Malaysian giant freshwater prawn (Macrobrachium rosenbergii) using next-generation sequencing. This provides a valuable resource for understanding prawn genetics, disease resistance, and aquaculture potential.

Area of Science:

  • Genomics
  • Aquaculture
  • Crustacean Biology

Background:

  • Gene discovery in Macrobrachium rosenbergii has been limited, hindering research despite its commercial importance.
  • Next-generation sequencing (NGS) technologies enable large-scale functional genomics data generation efficiently.

Purpose of the Study:

  • To generate a comprehensive transcriptome dataset for M. rosenbergii using RNA-sequencing.
  • To identify novel genes, non-coding RNAs, and potential molecular markers.
  • To elucidate gene functions related to various biological processes in different tissues.

Main Methods:

  • Whole transcriptome sequencing (RNA-seq) was performed on three tissue types: hepatopancreas, gill, and muscle.
  • De novo assembly of Illumina reads generated 102,230 unigenes.

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  • Sequence homology searches, in silico predictions, and KEGG pathway enrichment were employed.

    • Main Results:

    • Identified approximately 24% known and novel protein-coding genes, non-coding RNA, and repetitive elements.
    • Discovered simple sequence repeat markers linked to protein-coding genes.
    • Systematically represented differentially expressed genes and described tissue-specific functions (neuroactive regulation, metabolism, stress response).

    Conclusions:

    • This study represents the most extensive transcriptome data for freshwater prawns, offering significant functional genomics resources.
    • The findings support previous experimental studies and provide a foundation for future molecular research in M. rosenbergii.
    • Comparative analysis with model organisms opens avenues for exploring conserved biological entities between vertebrates and crustaceans.