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Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
Published on: April 11, 2016
Fast and robust method to determine phenoyl and acetyl esters of polysaccharides by quantitative ¹H NMR
Klaus G Neumüller1, Adriana Carvalho de Souza, Joep Van Rijn
1DSM Biotechnology Center, P.O. Box 1, 2600 MA Delft, The Netherlands.
Abstract:
The acetyl (AcE), feruloyl (FE), and p-coumaroyl (pCE) ester contents of different cereal and grass polysaccharides were determined by a quantitative ¹H NMR-based method. The repeatability and the robustness of the method were demonstrated by analyzing different plant polysaccharide preparations. Good sensitivity and selectivity for AcE, FE, and pCE were observed. Moreover, an optimized and easy sample preparation allowed for simultaneous quantification of AcE, FE, and pCE. The method is suitable for high-throughput analysis, and it is a good alternative for currently used analytical procedures. A comparison of the method presented to a conventional HPLC-based method showed that the results obtained are in good agreement, whereas the combination of the optimized sample preparation and analysis by the ¹H NMR-based methodology results in significantly reduced analysis time.
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