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Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells
Published on: May 26, 2021
Increased fibroblast growth factor 21 expression in high-fat diet-sensitive non-human primates (Macaca mulatta)
E B Nygaard1, C L Møller2, P Kievit3
11] Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, Denmark [2] Department of Diabetes NBEs and Obesity Biology, Novo Nordisk A/S, Måløv, Denmark.
International Journal of Obesity (2005)
|June 6, 2013
Summary
Fibroblast growth factor 21 (FGF21) levels increase with high-fat diets (HFDs). Maintaining FGF21 co-receptor β-klotho in white adipose tissue protected monkeys from metabolic dysfunction.
Area of Science:
- Metabolic regulation
- Endocrinology
- Primate models
Background:
- Fibroblast growth factor 21 (FGF21) is a key regulator of glucose and lipid metabolism.
- FGF21 administration lowers blood glucose in diabetic models.
- Elevated FGF21 levels are observed in obese and diabetic individuals.
Purpose of the Study:
- To investigate the role of FGF21 in diet-induced metabolic changes in rhesus macaques.
- To examine tissue-specific expression of FGF21 and its receptors in response to a high-fat diet (HFD).
- To differentiate metabolic responses between HFD-sensitive and HFD-resistant monkeys.
Main Methods:
- Adult male rhesus macaques were fed a high-fat diet (HFD) or standard diet (control).
- Plasma FGF21, lipids, and glucose-stimulated insulin secretion (GSIS) were measured.
- FGF21 and co-receptor β-klotho expression was analyzed in liver, pancreas, adipose tissue, and skeletal muscle.
Main Results:
- HFD induced obesity, dyslipidemia, and impaired glucose tolerance in sensitive monkeys.
- All HFD-fed monkeys showed increased plasma FGF21; sensitive monkeys had higher tissue expression.
- HFD-sensitive monkeys exhibited lower β-klotho expression in white adipose tissue (WAT).
Conclusions:
- High-fat diets alter FGF21 and receptor expression in a tissue-specific manner.
- Differential regulation of FGF21 occurs between HFD-sensitive and HFD-resistant individuals.
- Preserved β-klotho levels in WAT on HFD protect against dyslipidemia and hyperglycemia.
