Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Imaging micrometer-sized aerosol particles with digital holography.

Optics letters·2024
Same author

Measuring spectral extinction with digital holography.

Applied optics·2024
Same author

Measuring extinction with digital holography: noisy holograms.

Optics express·2024
Same author

Video-rate Raman-based metabolic imaging by Airy light-sheet illumination and photon-sparse detection.

Proceedings of the National Academy of Sciences of the United States of America·2023
Same author

The color of aerosol particles.

Scientific reports·2023
Same author

Electromagnetically induced modification of gold optical properties.

Optics express·2022

Related Experiment Video

Updated: May 10, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Backscatter digital holography of microparticles.

Nava R Subedi1, Nicholas B Fowler, Matthew J Berg

  • 1Department of Physics & Astronomy, Mississippi State University, Mississippi State, MS 39762, USA.

Optics Express
|June 6, 2013
PubMed
Summary

This study presents a new digital holographic imaging method for microparticles using backscattered light. This technique may offer enhanced sensitivity to particle surface details compared to traditional forward scatter methods.

More Related Videos

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
14:58

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters

Published on: June 2, 2010

Related Experiment Videos

Last Updated: May 10, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
14:58

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters

Published on: June 2, 2010

Area of Science:

  • Optics
  • Microscopy
  • Particle Imaging

Background:

  • Digital holography typically uses forward scattered light for imaging.
  • Characterizing microparticle surface details remains a challenge in microscopy.

Purpose of the Study:

  • To investigate digital holographic imaging of microparticles using backscattered light.
  • To explore the potential of backscatter holography for enhanced surface detail detection.

Main Methods:

  • Development of a digital holographic imaging technique utilizing backscattered light from microparticles.
  • Computational reconstruction of particle images from backscatter holograms.

Main Results:

  • Successful generation of reconstructed images of microparticles from backscatter holograms.
  • Demonstration of the feasibility of the backscatter holographic imaging approach.

Conclusions:

  • The proposed method offers a novel approach to digital holographic imaging of microparticles.
  • Backscatter holography shows promise for increased sensitivity to microparticle surface characteristics.