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Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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Spectrophotometry: Introduction01:16

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Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
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There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
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Related Experiment Video

Updated: Jul 1, 2025

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
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Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

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Measuring spectral extinction with digital holography.

Matthew J Berg, Killian Aleau, Romain Ceolato

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    |March 4, 2024
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    Summary
    This summary is machine-generated.

    Digital in-line holography (DIH) measures particle extinction cross sections contact-free. This technique accurately quantifies extinction for aerosols and other particles across a wide spectrum.

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    Area of Science:

    • Optical physics
    • Atmospheric science
    • Particle characterization

    Background:

    • Accurate measurement of particle extinction is crucial for climate modeling, visibility assessment, and remote sensing.
    • Digital in-line holography (DIH) offers a unique contact-free method for simultaneous particle imaging and extinction cross-section estimation.

    Purpose of the Study:

    • To investigate the measurement of particle extinction cross sections using DIH across a broad spectrum (440–1040 nm).
    • To assess the accuracy of DIH for various particle types, including a glass microsphere, volcanic ash, and iron(III) oxide.
    • To explore wavelength-dependent image evolution for distinguishing between absorption and scattering dominance.

    Main Methods:

    • Utilized a supercontinuum laser source for broadband illumination.
    • Employed digital in-line holography (DIH) to record holograms of stationary particles.
    • Integrated holographic data and extrapolated to determine extinction cross sections.

    Main Results:

    • Achieved extinction cross-section estimation with approximately 10% error across parts of the spectrum, and 20% error otherwise.
    • Observed wavelength-dependent changes in DIH-derived particle images.
    • Demonstrated the potential to differentiate between dominant absorption or scattering based on image evolution.

    Conclusions:

    • DIH is a viable technique for contact-free measurement of particle extinction cross sections with reasonable accuracy.
    • Wavelength-dependent holographic image analysis provides insights into particle optical properties (absorption vs. scattering).
    • Further development of DIH could enhance its application in atmospheric and material science research.