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Updated: May 10, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
Published on: March 15, 2019
Isaac: ultra-fast whole-genome secondary analysis on Illumina sequencing platforms
Come Raczy1, Roman Petrovski, Christopher T Saunders
1Illumina United Kingdom, Chesterford Research Park, Little Chesterford, Nr Saffron Walden, Essex, UK. craczy@illumina.com
Summary:
An ultrafast DNA sequence aligner (Isaac Genome Alignment Software) that takes advantage of high-memory hardware (>48 GB) and variant caller (Isaac Variant Caller) have been developed. We demonstrate that our combined pipeline (Isaac) is four to five times faster than BWA + GATK on equivalent hardware, with comparable accuracy as measured by trio conflict rates and sensitivity. We further show that Isaac is effective in the detection of disease-causing variants and can easily/economically be run on commodity hardware.
Availability:
Isaac has an open source license and can be obtained at https://github.com/sequencing.
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