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Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Related Experiment Video

Updated: May 10, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

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Optical coherenscopy based on phase-space tomography.

Alejandro Cámara1, José A Rodrigo, Tatiana Alieva

  • 1Universidad Complutense de Madrid, Facultad de Ciencias Físicas, Ciudad Universitaria s/n, Madrid 28040, Spain.

Optics Express
|June 6, 2013
PubMed
Summary

We developed a novel technique for fast, video-rate measurement and analysis of light

Area of Science:

  • Optics and Photonics
  • Quantum Optics
  • Optical Metrology

Background:

  • Partially coherent light offers advantages in imaging, beam shaping, and communications.
  • Characterizing spatial coherence is challenging due to its complex, high-dimensional nature.
  • Existing methods struggle with real-time, quantitative local coherence analysis without prior information.

Purpose of the Study:

  • To introduce a new technique for efficient experimental characterization of spatial coherence.
  • To enable fast, quantitative, and local analysis of partially coherent beams.
  • To simplify the process of understanding and utilizing light's coherence properties.

Main Methods:

  • Development of an automated optical setup for video-rate measurements.

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Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
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Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

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Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

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  • Implementation of phase-space tomographic coherenscopy for parallel data acquisition and processing.
  • Utilizing second-order statistics for spatial coherence analysis.

    • Main Results:

    • Demonstration of a technique enabling rapid, automated spatial coherence measurements.
    • Successful parallel acquisition, processing, and analysis of coherence data.
    • Significant simplification of spatial coherence analysis compared to traditional methods.

    Conclusions:

    • The proposed technique effectively addresses the challenges in characterizing spatial coherence.
    • This method facilitates the development of new tools leveraging light coherence.
    • Opens new avenues for applications in imaging, communications, and random medium monitoring.