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Updated: May 10, 2026

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
Published on: November 18, 2022
Two dimensional interferometric optical trapping of multiple particles and Escherichia coli bacterial cells using a
Ashleigh L Barron1, Ajoy K Kar, Thomas J Aspray
1Institute of Photonics and Quantum Sciences, School of Engineering and Physical Sciences, Heriot Watt University, Edinburgh, UK. ab461@hw.ac.uk
Abstract:
Two dimensional interferometric trapping of multiple microspheres and Escherichia coli has been demonstrated using a multicore fiber lensed with an electric arc fusion splicer. Light was coupled evenly into all four cores using a diffractive optical element. The visibility of the fringes and also the appearance of the lattice can be altered by rotating a half wave-plate. As a result the particles can be manipulated from one dimensional trapping to two dimensional trapping or a variety of different two dimensional arrangements. The ability to align bacterial populations has potential application for quorum sensing, floc and biofilm and, metabolic co-operation studies.
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