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Related Experiment Video

Updated: May 10, 2026

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
07:16

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Published on: August 18, 2022

Decrease in cytosine methylation at CpG island shores and increase in DNA fragmentation during zebrafish aging.

Nobuyoshi Shimoda1, Toshiaki Izawa, Akio Yoshizawa

  • 1Department of Regenerative Medicine, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, 36-3 Gengo, Morioka, Ōbu, Aichi, 474-8522, Japan, shimoda@ncgg.go.jp.

Age (Dordrecht, Netherlands)
|June 6, 2013
PubMed
Summary

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Zebrafish exhibit age-related DNA hypomethylation in somatic cells, particularly at CpG island shores, mirroring mammalian aging. This epigenetic shift and increased genome cleavage suggest a role in senescence and apoptosis.

Area of Science:

  • Epigenetics
  • Comparative genomics
  • Aging research

Background:

  • Age-related DNA methylation changes are known in mammals.
  • The generality of these epigenetic changes across vertebrates is not well understood.
  • Understanding vertebrate aging mechanisms is crucial for senescence research.

Purpose of the Study:

  • To investigate age-related DNA methylation patterns in zebrafish (Danio rerio).
  • To determine if hypomethylation occurs in specific genomic regions associated with aging.
  • To explore the role of epigenetics in vertebrate senescence.

Main Methods:

  • Analysis of methylcytosine levels in zebrafish somatic and germ cells across different ages.
  • Identification of genomic regions undergoing age-dependent hypomethylation, focusing on CpG island shores.

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Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo
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Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo

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Related Experiment Videos

Last Updated: May 10, 2026

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
07:16

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Published on: August 18, 2022

Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo
10:10

Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo

Published on: July 15, 2016

  • Assessment of genome integrity and cleavage in aging zebrafish somatic cells.

    • Main Results:

    • Zebrafish somatic cells showed a gradual loss of methylcytosine with age, unlike male germ cells.
    • Age-dependent hypomethylation was prominent in CpG island shores, a pattern observed in aging humans.
    • Hypomethylated regions in oocytes were re-methylated in fertilized eggs, indicating epigenetic resetting.
    • Increased genome cleavage to oligonucleosome length was observed in 12-month-old zebrafish, suggesting apoptosis.

    Conclusions:

    • Zebrafish display age-related DNA hypomethylation, supporting its conserved nature in vertebrates.
    • Epigenetic resetting upon fertilization ensures a methylated genome for new generations.
    • Elevated genome cleavage in aged zebrafish suggests increased apoptosis during senescence.