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Related Concept Videos

The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

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Related Experiment Video

Updated: May 10, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Density-dependent cooperative non-specific binding in solid-phase SELEX affinity selection.

Abdullah Ozer1, Brian S White, John T Lis

  • 1Department of Molecular Biology and Genetics, Cornell University, Biotechnology Building, Ithaca, NY 14853,USA.

Nucleic Acids Research
|June 6, 2013
PubMed
Summary
This summary is machine-generated.

Cooperative binding of RNA aptamers to multiple targets enhances affinity. This finding improves ligand enrichment methods like SELEX (Systematic Evolution of Ligands by Exponential Enrichment) and microfluidic devices.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biophysics

Background:

  • Non-specific binding limits ligand enrichment in affinity selection.
  • Solid-phase binding affinity can exceed solution-phase affinity due to cooperativity.

Purpose of the Study:

  • To investigate cooperative binding of ligands to immobilized targets.
  • To develop a model explaining density-dependent cooperative binding.

Main Methods:

  • Systematic Evolution of Ligands by Exponential Enrichment (SELEX) experimental design.
  • Statistical mechanical modeling of cooperative binding.

Main Results:

  • RNA aptamers demonstrated concurrent binding to up to four immobilized peptide targets.
  • Effective binding affinity increased by two orders of magnitude due to cooperativity.
  • A novel model quantitatively explained density-dependent cooperative binding.

Conclusions:

  • Cooperative binding significantly enhances ligand affinity on solid supports.
  • Target immobilization and density are critical for optimizing ligand enrichment.
  • Results have implications for SELEX and microfluidic purification device performance.