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Related Concept Videos

Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
Nucleic acids02:43

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

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Related Experiment Video

Updated: May 10, 2026

An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

Engineering RNA-binding proteins for biology.

Yu Chen1, Gabriele Varani

  • 1Department of Biochemistry, University of Washington, Seattle, WA 98195-1700, USA. chenyu@u.washington.edu

The FEBS Journal
|June 8, 2013
PubMed
Summary
This summary is machine-generated.

Engineered RNA-binding proteins offer precise gene regulation tools. Tailoring protein specificity enhances biochemical research and therapeutic potential.

Keywords:
RNA recognition motifRNA-binding domainsRNA-binding proteinscomputational designin vitro evolutionphage displayprotein engineeringprotein-RNA interactionsyeast three-hybrid systemzinc finger

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Novel RNA-Binding Proteins Isolation by the RaPID Methodology
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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

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Related Experiment Videos

Last Updated: May 10, 2026

An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • RNA-binding proteins (RBPs) are crucial for gene expression regulation.
  • RBPs often possess modular structures with common domains for RNA recognition.
  • Engineering RBPs allows for targeted RNA metabolism control.

Purpose of the Study:

  • To review RNA-binding domains suitable for engineering specificity.
  • To compare protein engineering and design methods for RBPs.
  • To discuss future applications of designer RBPs.

Main Methods:

  • Analysis of structural basis for RNA recognition by various domains.
  • Comparison of different protein engineering strategies.
  • Literature review on PUF protein engineering.

Main Results:

  • Certain RNA-binding domains are more amenable to specificity engineering.
  • Modular assembly of domains allows for tailored RNA-binding proteins.
  • Progress in engineering PUF proteins demonstrates feasibility.

Conclusions:

  • Designer RNA-binding proteins hold promise for research and therapeutics.
  • Understanding structural recognition is key to engineering specificity.
  • Further development of protein design methods is ongoing.