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Related Concept Videos

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

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Related Experiment Video

Updated: May 10, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Multimodality imaging of RNA interference.

T R Nayak1, L K Krasteva, W Cai

  • 1Department of Radiology, University of Wisconsin - Madison, Madison, WI 53705-2275, USA.

Current Medicinal Chemistry
|June 11, 2013
PubMed
Summary
This summary is machine-generated.

In vivo imaging advances RNA interference (RNA) therapy by tracking small interfering RNA (siRNA) delivery and efficacy. Developing non-invasive imaging strategies is crucial for clinical translation of RNAi therapeutics.

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Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection
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Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection

Published on: January 26, 2019

Related Experiment Videos

Last Updated: May 10, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection
06:44

Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection

Published on: January 26, 2019

Area of Science:

  • Molecular biology and genetics
  • Biomedical imaging
  • Therapeutic drug development

Background:

  • RNA interference (RNAi) using small interfering RNA (siRNA) and short hairpin RNA (shRNA) offers gene knockdown potential.
  • Clinical application of RNAi is hindered by inefficient delivery of siRNA/shRNA molecules.
  • Molecular imaging plays a vital role in assessing RNAi delivery, biodistribution, pharmacokinetics, and therapeutic outcomes.

Purpose of the Study:

  • To review the current applications of in vivo molecular imaging techniques in RNAi research.
  • To highlight the importance of imaging for evaluating siRNA/shRNA delivery and therapeutic effects.
  • To discuss future directions for non-invasive imaging strategies in RNAi clinical translation.

Main Methods:

  • Review of existing literature on molecular imaging techniques used in RNAi studies.
  • Inclusion of techniques such as bioluminescence/fluorescence imaging, MRI/MRS, PET, and SPECT.
  • Discussion of combined multimodality imaging approaches.

Main Results:

  • Various molecular imaging modalities have been successfully employed to monitor RNAi processes in vivo.
  • Imaging allows for the evaluation of siRNA/shRNA delivery, biodistribution, and pharmacokinetic profiles.
  • Current imaging methods provide insights into the therapeutic efficacy of RNAi interventions.

Conclusions:

  • Advancements in non-invasive imaging are critical for the clinical translation of RNAi therapies.
  • Further validation is needed to correlate carrier biodistribution with siRNA/shRNA distribution.
  • Multimodality imaging approaches are essential for comprehensive monitoring of RNAi delivery and gene silencing.