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Related Experiment Video

Updated: May 10, 2026

Creation of Colonic Anastomosis in Mice
07:22

Creation of Colonic Anastomosis in Mice

Published on: January 17, 2019

Colon anastomotic leakage: improving the mouse model.

Hans-Christian Pommergaard1, Michael Patrick Achiam, Jacob Rosenberg

  • 1Department of Surgical Gastroenterology D, Herlev Hospital, Center for Perioperative Optimization, University of Copenhagen, Herlev Ringvej 75, 2730, Herlev, Denmark, HCPommergaard@gmail.com.

Surgery Today
|June 11, 2013
PubMed
Summary

This study developed a mouse model for colon anastomotic leakage, showing a 40% leakage rate in the intervention group versus 0% in controls. This model accurately reflects human clinical outcomes.

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Area of Science:

  • Surgical Research
  • Animal Models
  • Gastrointestinal Surgery

Background:

  • Colon anastomotic leakage is a frequent and severe surgical complication.
  • Animal models are crucial for understanding leakage mechanisms and testing preventive strategies.

Purpose of the Study:

  • To develop an optimal mouse model for clinical colon anastomotic leakage.
  • To create a technically insufficient anastomosis model in mice.

Main Methods:

  • 110 mice were used across pilot studies and experiments.
  • Surgical techniques and analgesia were refined, with buprenorphine used for pain relief in the final model.
  • Absorbable sutures were varied, with 8 in controls and 4 in the intervention group for the final experiment.

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Last Updated: May 10, 2026

Creation of Colonic Anastomosis in Mice
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Main Results:

  • The final model (Experiment B) showed significantly higher clinical anastomotic leakage in the intervention group (40%) compared to controls (0%) (p = 0.003).
  • Animals in the intervention group experienced greater weight loss and lower wellness scores (p < 0.001).
  • Anastomotic breaking strength did not differ significantly between groups (p = 0.091).

Conclusions:

  • The developed mouse model effectively mimics human colon anastomotic leakage.
  • The model holds high clinical relevance due to similar causes, incidence, and manifestations as observed in humans.