Related Experiment Video
Updated: May 10, 2026

Glycan Profiling of Plant Cell Wall Polymers using Microarrays
Published on: December 17, 2012
Preliminary characterization of proteoglycan from Hyriopsis cumingii
De-Liang Qiao1, Lin Wang, Jun Liu
1College of Biological and Pharmaceutical Engineering, West Anhui University, Liu'an 237012, China. qiaodll@wxc.edu.cn
Objective:
To study the preliminary characterizations of Hyriopsis cumingii proteoglycan (HCPG).
Methods:
The content of carbohydrate and protein were measured by spectrophotometry. FTIR spectrum was used to analyze the functional groups. Relative molecular mass and amino acid composition were detected by HPLC. GC was utilized to determine the monosaccharide composition. The glycopeptide linkage-bond was detected by using the method of beta-elimination reaction.
Results:
In HCPG,the content of carbohydrate and protein was 80.06% and 9.42%, respectively. FTIR spectrum showed the characteristic absorptions of polysaccharides and protein. Relative molecular mass of HCPG, determined by size-exclusive HPLC, was 503.1 kDa. GC spectra demonstrated that polysaccharide of HCPG was composed of rhamnose, fucose, mannose, glucose and galactose with a molar ratio of 13.80: 4.51: 7.70: 64.92 : 9.07. Fourteen amino acids (13 known and one unknown) have been detected by pre-column derivation HPLC. From beta-elimination reaction, peptide chain was attached to the carbohydrate chain by O-glycosidic bond.
Conclusion:
Basic characterizations of HCPG have been determined preliminarily.
Related Concept Videos
Proteoglycans
Glycosaminoglycans
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...
Matrix Proteoglycans and Glycoproteins

