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[Study on MEKC-DAD fingerprint of Scutellariae radix]
Le Han1, Qiao-Xia Chen, Xun-Hong Liu
1Jiangsu Key Laboratory of Chinese Medicines Processing, Nanjing University of Chinese Medicine, Nangjing 210046, China. winner2243@sohu.com
Objective:
To establish the analytical method for the fingerprint of Scutellariae Radix by MEKC-DAD and compare the fingerprints of Scutellariae Radix, Scutellariae Radix Stir-baked and Scutellariae Radix Green.
Methods:
Based on the mode of micellar electrokinetic chromatography, 40 mmol/L sodium hydrogen phosphate, 15 mmol/L sodium borate, 40 mmol/L SDS, 15% acetonitrile, 7.5% propyl alcohol were selected for the running buffer (pH 8.4). The separation voltage was 20 kV and the detection wavelength was set at 280 nm. Baicalin was used as reference standard, the chromatographic fingerprint was established.
Results:
MEKC-DAD fingerprint with 9 main peaks was established preliminarily. Regarding to the fingerprints of Scutellariae Radix and its processed products, the samples before and after storage moisture, there were obvious differences in the relative areas of common peaks.
Conclusion:
The method is reliable, accurate and can be used for quality control of Scutellariae Radix.
