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[Optimization of extraction of Clerodendranthus spicatus using response surface methodology]
1Yunnan Branch, Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Jinghong 666100.
Objective:
To obtain the optimal conditions of extraction of Clerodendranthus spicatus and provide the theoretical foundation for its further processing and utilization.
Methods:
On the basis of the single factor test, response surface methodology was applied to optimize the extraction conditions.
Results:
The results showed that extraction time, water-feed ratio, ethanol concentration and extraction temperature all had significant effects on the extraction rate of polysaccharides. The optimal extraction time was 3h, solid-liquid ratio was 50:1, ethanol concentration was 30% and extraction temperature was 80 degrees C. Under these optimized conditions, the extraction rate was 27.71%.
Conclusion:
The extraction technology is simple, reliable and highly predictive.
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Response Surface Methodology
The process of RSM involves several key steps:
Methods of Medium Optimization

