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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
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The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...

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Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy
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Destabilization induced by electropermeabilization analyzed by atomic force microscopy.

Louise Chopinet1, Charles Roduit, Marie-Pierre Rols

  • 1CNRS, LAAS, 7 avenue du colonel Roche, F-31400 Toulouse, France.

Biochimica Et Biophysica Acta
|June 13, 2013
PubMed
Summary
This summary is machine-generated.

Electropermeabilization uses electric pulses to deliver molecules into cells. Atomic force microscopy revealed membrane surface rippling and a 40% decrease in elasticity, suggesting internal cell effects during this process.

Keywords:
AFMAtomic Force MicroscopyAtomic force microscopeCHOChinese Hamster Ovary CellCytoskeletonEPElectropermeabilizationElectroporationLiving cellsQI™Quantitative Imaging™StiffnessYMYoung Modulus

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Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

Published on: July 22, 2015

Area of Science:

  • Cell biology
  • Biophysics
  • Nanotechnology

Background:

  • Electropermeabilization is a technique for molecule delivery into cells using electric fields.
  • The precise mechanisms of plasma membrane destabilization during electropermeabilization remain poorly understood.
  • Understanding these mechanisms is crucial for optimizing therapeutic applications.

Purpose of the Study:

  • To visualize and quantify membrane changes during electropermeabilization using atomic force microscopy (AFM).
  • To investigate the effects of electric field pulses on cell membrane organization and elasticity.
  • To explore potential links between membrane changes and internal cellular structures like the cytoskeleton.

Main Methods:

  • Utilized atomic force microscopy (AFM) to image cell membranes at high resolution.
  • Measured local membrane elasticity before and after electropermeabilization.
  • Applied electropermeabilization to both fixed and living Chinese Hamster Ovary (CHO) cells.

Main Results:

  • Direct visualization of transient membrane surface rippling induced by electric pulses.
  • Quantified a significant decrease in membrane elasticity by approximately 40%.
  • Observed effects consistent with internal cellular destabilization, potentially involving the cytoskeleton.

Conclusions:

  • AFM is a valuable tool for studying the biophysical processes of electroporation in living cells without staining.
  • Electropermeabilization induces not only membrane surface changes but also affects the cell's internal structure.
  • These findings provide new insights into the fundamental mechanisms of electropermeabilization.