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Related Concept Videos

Vaccine Production01:23

Vaccine Production

Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...
Vaccinations01:51

Vaccinations

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Related Experiment Video

Updated: May 10, 2026

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
08:36

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

Current progress in dengue vaccines.

Shu-Wen Wan1, Chiou-Feng Lin, Shuying Wang

  • 1Department of Microbiology and Immunology, National Cheng Kung University Medical College, Tainan, Taiwan.

Journal of Biomedical Science
|June 14, 2013
PubMed
Summary
This summary is machine-generated.

Dengue fever, a viral disease transmitted by mosquitoes, presents risks ranging from mild illness to severe conditions like dengue hemorrhagic fever. Research is ongoing for dengue vaccines, focusing on safety and efficacy by addressing immunopathogenic complications.

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Area of Science:

  • * Virology and immunology
  • * Vector-borne diseases
  • * Vaccine development

Background:

  • * Dengue viruses (DENV) cause self-limited dengue fever (DF) or severe dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS).
  • * Severe DENV disease involves vascular leakage, thrombocytopenia, and hemorrhage, with complex underlying mechanisms.
  • * Immunopathological factors contribute significantly to dengue symptom development.

Purpose of the Study:

  • * To review the current landscape of dengue vaccine development.
  • * To highlight the clinical manifestations and unresolved mechanisms of severe dengue.
  • * To emphasize the importance of considering immunopathogenic complications for dengue vaccine safety and efficacy.

Main Methods:

  • * Review of existing literature on dengue virus, disease pathogenesis, and vaccine candidates.
  • * Analysis of different vaccine types under development (live attenuated, chimeric, inactivated, recombinant, DNA, subunit).
  • * Evaluation of clinical and preclinical data for vaccine candidates.

Main Results:

  • * Four DENV serotypes exist, causing a spectrum of illness.
  • * Multiple dengue vaccine candidates are in various stages of development and clinical evaluation.
  • * Live attenuated and live chimeric virus vaccines are currently in clinical trials.

Conclusions:

  • * No licensed dengue vaccine is currently available.
  • * Vaccine development must address potential immunopathogenic issues like antibody-mediated enhancement and autoimmunity.
  • * Further research is crucial for developing safe and effective dengue vaccines.