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Related Concept Videos

Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Chromatography: Introduction01:10

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
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Analyte Adsorption and Distribution01:09

Analyte Adsorption and Distribution

In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and solvents...
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Dialysis

Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...

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Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

Distributed pore model for bio-molecule chromatography.

Bertrand Coquebert de Neuville1, Abhijit Tarafder, Massimo Morbidelli

  • 1Institute for Chemical and Bioengineering, Department of Chemistry and Applied Bioscience, ETH Zurich, 8093 Zurich, Switzerland.

Journal of Chromatography. A
|June 14, 2013
PubMed
Summary
This summary is machine-generated.

A new pore model accounts for protein adsorption effects in chromatography, improving predictions of dynamic binding capacity. This model offers a more accurate simulation of mass transport in porous particles.

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Published on: November 25, 2020

Area of Science:

  • Biochemical Engineering
  • Chromatography Science
  • Protein Separation Technology

Background:

  • Protein chromatography faces challenges due to comparable sizes of proteins and adsorbent pores.
  • Protein adsorption significantly reduces pore size, impacting pore accessibility and mass transport dynamics.

Purpose of the Study:

  • To develop a novel model that incorporates pore size distribution and protein-induced pore shrinkage.
  • To accurately describe mass transport and adsorption within porous particles during protein chromatography.
  • To validate the new model against the General Rate Model (GRM) and experimental data.

Main Methods:

  • Developed a new pore model considering stationary phase pore size distribution and pore shrinkage.
  • Demonstrated the model's equivalence to the General Rate Model (GRM) under diluted conditions.
  • Applied the model to simulate lysozyme breakthrough experiments.

Main Results:

  • The new pore model accurately simulates protein adsorption and mass transport.
  • The model successfully predicts dynamic binding capacity (DBC) from static capacity measurements.
  • Model performance was validated against GRM and experimental lysozyme breakthrough data.

Conclusions:

  • The developed pore model provides a more accurate representation of protein chromatography processes.
  • This model enhances the prediction of dynamic binding capacity, crucial for process optimization.
  • The findings offer improved insights into mass transport phenomena in porous adsorbents.