Substrate specificity and interferences of a direct-electron-transfer-based glucose biosensor.

Area of Science:

• Electrochemistry
• Biosensors
• Biotechnology

Background:

• Electrochemical glucose monitoring relies on signal transduction for electron transfer.
• Existing sensors face challenges with interfering substances.
• Direct electron transfer offers a new approach.

Purpose of the Study:

• To develop and evaluate a glucose biosensor employing direct electron transfer.
• To assess the biosensor's response to interfering substances.
• To explore the potential for continuous glucose monitoring applications.

Main Methods:

• Immobilization of cellobiose dehydrogenase (CDH) enzyme on a carbon electrode.
• Utilizing flow-injection analysis, linear sweep, and chronoamperometry for measurements.
• Testing the biosensor response to glucose and interfering compounds.

Main Results:

• Stable glucose signal achieved with a sensitivity of 0.21 µA mM⁻¹ cm⁻².
• Minimal signal deviation (<5%) observed in the presence of interfering substances.
• Successful operation at a low polarization potential (-100 mV vs. Ag/AgCl).

Conclusions:

• Direct electron transfer is a viable and novel principle for glucose biosensors.
• The developed biosensor exhibits significant reduction in interference.
• This technology shows promise for advanced continuous glucose monitoring systems.