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Testing for isoniazid. An evaluation of the Arkansas method.
D E Schraufnagel1, R Stoner, E Whiting
1Veterans Administration West Side Medical Center, Chicago.
Chest
|August 1, 1990
Summary
The Potts-Cozart test reliably detects isoniazid in urine, aiding tuberculosis treatment compliance. This simple, inexpensive method also identifies nicotine, without confusing results.
Area of Science:
- Clinical Chemistry
- Pharmacology
- Public Health
Background:
- Patient compliance is crucial for tuberculosis (TB) treatment and elimination.
- Urine drug testing is a method to assess patient compliance.
- The Potts-Cozart test is a colorimetric method for detecting isoniazid in urine, primarily used in Arkansas.
Purpose of the Study:
- To evaluate the reliability and accuracy of the Potts-Cozart test for detecting isoniazid in urine.
- To investigate variations in the test's colorimetric results.
- To assess the test's utility in a clinical setting for monitoring TB medication adherence.
Main Methods:
- Blind testing of over 200 urine samples from TB clinic patients.
- Inclusion of control urine samples from substance abuse and Veterans Medical Center clinics.
- Absorption spectroscopy was used to analyze color variations and identify interfering substances.
Main Results:
- The Potts-Cozart test demonstrated no false-positives in the tested samples.
- Three patients presumed to be on medication showed normal urinalysis, suggesting noncompliance.
- Nicotine was identified as a substance causing color variation, but did not interfere with isoniazid detection.
Conclusions:
- The Potts-Cozart test is a simple, quick, inexpensive, and reliable method for detecting isoniazid in urine.
- The test can aid in improving patient compliance for tuberculosis treatment.
- The test also has the capability to detect nicotine and its metabolites.