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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: May 10, 2026

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR
10:23

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Published on: February 12, 2018

Plasma processing conditions substantially influence circulating microRNA biomarker levels.

Heather H Cheng1, Hye Son Yi, Yeonju Kim

  • 1Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America.

Plos One
|June 14, 2013
PubMed
Summary

Processing blood for cell-free microRNAs (miRNAs) significantly impacts measurements due to platelet contamination. Additional centrifugation steps are crucial for accurate miRNA biomarker studies.

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Last Updated: May 10, 2026

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR
10:23

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Published on: February 12, 2018

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MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening

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Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein
09:48

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Published on: July 27, 2016

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biomarker Discovery

Background:

  • Circulating cell-free microRNAs (miRNAs) are potential biomarkers.
  • Previous research indicated plasma miRNAs are often blood cell-derived.
  • Elevated miRNA levels in lung cancer cases correlated with platelet counts.

Purpose of the Study:

  • To investigate the impact of plasma processing on circulating miRNA measurements.
  • To determine if platelet and microparticle contamination affects miRNA quantification.
  • To establish optimal processing conditions for reliable miRNA biomarker studies.

Main Methods:

  • Matched plasma from healthy individuals was processed using differential centrifugation and filtration.
  • Stepwise processing was employed to isolate effects of centrifugation and filtration.
  • miRNA profiling was performed on processed plasma samples.

Main Results:

  • Plasma processing alone substantially affected 72% of detectable miRNAs.
  • Significant variation (4-30x to >1,000x) in miRNA expression was observed due to processing.
  • Platelet contamination was identified as the primary cause of variation, persisting with standard protocols.

Conclusions:

  • Standard plasma processing protocols introduce significant confounding variation in miRNA measurements.
  • Additional centrifugation steps can effectively reduce platelet contamination, even in long-term archived samples.
  • Implementing enhanced processing protocols is essential for accurate circulating miRNA biomarker studies.