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Updated: May 10, 2026

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
Published on: October 26, 2016
BSA and fibrinogen adsorption on chitosan/κ-carrageenan polyelectrolyte complexes
Thiane N Carneiro1, Denise S Novaes, Rodrigo B Rabelo
1Departamento de Engenharia Química, Universidade Federal do Ceará, Centro de Tecnologia, Av. Mister Hull, s/n, Campus do Pici, Bloco 709 Pici, CEP 60455-760 Fortaleza, CE, Brazil.
Abstract:
PECs of chitosan/κ-carrageenan are prepared in three different volumetric rations. The complex formation is characterized in order to evaluate the blending formation. Blood compatibility is evaluated by protein adsorption (BSA and fibrinogen) and PEC toxicities are determined with fibroblast cell viability and proliferation. The swelling degree of PECs decreases when the amount of chitosan increases. Due to the linked film formation, PECs decrease BSA adsorption and increase fibrinogen adsorption when compared to the pristine chitosan and κ-carrageenan films. Although pristine chitosan and κ-carrageenan films produced similar cell expansion and viability, the PEC 50:50 vol% chitosan/κ-carrageenan PEC may be acceptable as a new scaffold for cell therapies, due to their effect on cell survival.

