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Published on: May 31, 2019
Determination of antimycin-A in water by liquid chromatographic/mass spectrometry: single-laboratory validation
Jeffry A Bernardy1, Terrance D Hubert, Jacob M Ogorek
1U.S. Geological Survey, Upper Midwest Environmental Sciences Center, 2630 Fanta Reed Rd, La Crosse, WI 54603, USA. jbernardy@usgs.gov
Abstract:
An LC/MS method was developed and validated for the quantitative determination and confirmation of antimycin-A (ANT-A) in water from lakes or streams. Three different water sample volumes (25, 50, and 250 mL) were evaluated. ANT-A was stabilized in the field by immediately extracting it from water into anhydrous acetone using SPE. The stabilized concentrated samples were then transported to a laboratory and analyzed by LC/MS using negative electrospray ionization. The method was determined to have adequate accuracy (78 to 113% recovery), precision (0.77 to 7.5% RSD with samples > or = 500 ng/L and 4.8 to 17% RSD with samples < or = 100 ng/L), linearity, and robustness over an LOQ range from 8 to 51 600 ng/L.
