Temperature controlled machine perfusion system for liver.

Area of Science:

• Transplantation immunology
• Organ preservation technologies
• Surgical innovation

Background:

• Static cold storage limits organ viability and transplant success.
• Machine perfusion (MP) offers superior organ preservation compared to static cold storage.
• Developing advanced MP systems is crucial for utilizing extended criteria and donation after cardiac death (DCD) organs.

Purpose of the Study:

• To introduce and evaluate a novel machine perfusion preservation system (NES-01).
• To assess the efficacy of NES-01 in preserving porcine liver grafts.
• To determine the utility of NES-01 in evaluating graft viability for functional recovery.

Main Methods:

• Development of a new machine perfusion system (NES-01) capable of controlling perfusate temperature from hypothermic to subnormothermic conditions.
• Application of the NES-01 system for the preservation of porcine liver grafts.
• Evaluation of graft viability and potential for functional recovery using the NES-01 system.

Main Results:

• The NES-01 system successfully preserved porcine liver grafts.
• The system demonstrated potential for evaluating graft viability for recovery.
• Controlled hypothermic to subnormothermic perfusion was achieved.

Conclusions:

• The novel NES-01 machine perfusion system is effective for liver graft preservation.
• This system aids in assessing graft viability, crucial for improving organ transplant success.
• The technology holds promise for organs from challenging donor sources, including DCD donors.