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Related Experiment Video

Updated: May 10, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Differences in alloimmune response between elderly and young mice.

B K Book¹, M A Volz, E K Ward

¹Department of Surgery, Indiana University School of Medicine, Indianapolis, Indiana. bbook@iupui.edu

Transplantation Proceedings

|June 18, 2013

Summary

Older mice showed significantly reduced alloantibody responses to new antigens compared to younger mice. This suggests age-related immune differences that may impact transplant outcomes.

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Last Updated: May 10, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

Area of Science:

Immunology
Transplantation Immunology
Gerontology

Background:

The aging population is increasing the upper age of renal transplant recipients.
Age-dependent immune responsiveness to novel antigens requires further investigation.
Understanding these age-related immune differences is crucial for optimizing transplant strategies.

Purpose of the Study:

To investigate age-related differences in immune response to neoalloantigens using a mouse model.
To compare alloantibody production in elderly versus young mice after immunization with foreign antigens.

Main Methods:

Transgenic mice were immunized with splenocytes, and plasma samples were collected over two months.
Alloantibody levels were measured using flow cytometric crossmatch and median fluorescence intensity.
Elderly (42-103 weeks) and young (11-15 weeks) mice were compared.

Main Results:

No significant difference in baseline alloantibody levels between elderly and young mice.
Younger mice exhibited significantly higher alloantibody responses at both 1 month (52.9 vs 5.12) and 2 months (109.38 vs 21.97) post-immunization.
These differences in alloantibody response were statistically significant (P < .0024 at 2 months).

Conclusions:

Older mice demonstrated significantly decreased immune responses to new alloantigen stimulation compared to younger mice.
This animal model can be utilized to study age-related immune refractoriness to antigen signaling.
Clinical immunosuppression protocols may need adaptation for the aged population due to potentially diminished immune responses.