Related Experiment Video

Updated: May 10, 2026

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment
10:59

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Published on: November 23, 2017

Plasminogen activator inhibitor-1 levels and activity decrease after intervention in patients with critical limb

M Björck1, M Lepkowska Eriksson, A Bylock

  • 1Department of Surgical Sciences, Section of Vascular Surgery, Uppsala University, Uppsala, Sweden. martin@bjorck.pp.se

European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery
|June 18, 2013
PubMed

Insights

Peripheral arterial occlusive disease (PAOD) patients undergoing treatment showed reduced plasminogen activator inhibitor-1 (PAI-1) levels post-intervention. This suggests a potential decrease in the prothrombotic state associated with critical limb ischaemia (CLI).

Area of Science:

  • Vascular Medicine
  • Thrombosis Research
  • Biomarker Analysis

Background:

  • Peripheral arterial occlusive disease (PAOD), especially critical limb ischaemia (CLI), is linked to a high risk of thrombotic events.
  • Elevated plasminogen activator inhibitor-1 (PAI-1) levels are hypothesized to contribute to a prothrombotic state in CLI patients.

Purpose of the Study:

  • To determine PAI-1 levels in patients with acute or chronic PAOD/CLI.
  • To investigate the impact of conservative, endovascular, or open surgical treatments on PAI-1 levels.

Main Methods:

  • Study included 32 patients (median age 74 years) with PAOD/CLI, undergoing various treatments.
  • Biomarkers, including PAI-1 and C-reactive protein (CRP), and ankle brachial index (ABI) were measured pre- and post-intervention.
  • Duplex ultrasound assessed vessel patency.

Main Results:

  • Ankle pressure and ABI significantly improved post-intervention (p < .001).
  • CRP levels transiently increased, while PAI-1 antigen and activity decreased from day 6 onwards post-intervention (p < .05).
  • Significant individual variability in PAI-1 levels was observed, with a notable percentage of patients exhibiting elevated PAI-1 at baseline and early post-intervention.

Conclusions:

  • Endovascular and open surgical treatments improved hemodynamic parameters in CLI patients.
  • Interventions led to a transient CRP increase and a sustained reduction in PAI-1 levels and activity.
  • The findings suggest a potential modulation of the prothrombotic state following treatment for CLI.
Abstract

Keywords:
Critical limb ischaemia (CLI)InflammationPeripheral arterial occlusive disease (PAOD)Plasminogen activator inhibitor-1 (PAI-1)ThrombosisTreatment

More Related Videos

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well08:01

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

Related Concept Videos

Peripheral Artery Disease III: Interprofessional Care01:27

Peripheral Artery Disease III: Interprofessional Care

Peripheral Artery Disease (PAD) is characterized by narrowed arteries that diminish blood flow to the extremities. Effective management of PAD requires an interprofessional approach involving various healthcare professionals. The critical aspects of interprofessional care for PAD patients focus on risk factor modification, drug therapy, exercise therapy, nutrition therapy, critical limb ischemia care, and interventional radiology and surgical procedures.The primary treatment goal for PAD...
Venous Thrombosis III: Interprofessional Care01:29

Venous Thrombosis III: Interprofessional Care

Venous thrombosis requires effective prevention and treatment strategies to improve patient outcomes and reduce potential complications.Prevention StrategiesHealthcare providers must prioritize preventing venous thromboembolism (VTE) for all adult patients upon admission. Interventions depend on bleeding and thrombosis risk, medical history, current medications, diagnoses, planned procedures, and patient preferences. Patients on bed rest should change positions every two hours and, if not...
Clot Retraction and Fibrinolysis01:16

Clot Retraction and Fibrinolysis

After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
Peripheral Artery Disease V: Postoperative Nursing Management01:23

Peripheral Artery Disease V: Postoperative Nursing Management

During the postoperative period, it is crucial to focus on maintaining circulation, identifying and managing potential complications, and planning for discharge.Nursing AssessmentVital signs monitoring: Regularly monitor vital signs, including blood pressure, heart rate, respiratory rate, and temperature, to detect early signs of complications such as bleeding and infection.Circulation assessment: Monitor pulses, perform Doppler assessments, and check capillary refill, color, temperature, and...
Peripheral Artery Disease I: Introduction01:30

Peripheral Artery Disease I: Introduction

Peripheral artery disease (PAD) predominantly results from atherosclerosis, which involves the accumulation of fatty deposits, or plaques, within the walls of arteries. This causes them to narrow and harden, significantly reducing blood flow. PAD predominantly affects the legs, particularly the arteries supplying the thighs and calves. In rare cases, it may involve other arteries, including those in the arms.Etiology of PAD:The principal cause of PAD is atherosclerosis, which results from fatty...