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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Updated: May 10, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

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Published on: September 5, 2019

Single-molecule detection: Breaking the concentration barrier

Philip Tinnefeld¹

¹Institute for Physical and Theoretical Chemistry, TU Braunschweig, Braunschweig University of Technology, 38106 Braunschweig, Germany. p.tinnefeld@tu-braunschweig.de

Nature Nanotechnology

|June 18, 2013

Summary

No abstract available in PubMed .

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