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Updated: May 10, 2026

Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations
Published on: January 10, 2015
Resolvin E1 maintains macrophage function under cigarette smoke-induced oxidative stress
Rina Takamiya1, Koichi Fukunaga, Makoto Arita
1Department of Biochemistry, School of Medicine, Keio University, Japan ; Division of Pulmonary Medicine, Department of Medicine, School of Medicine, Keio University, Japan ; Systems Glycobiology Research Group, Advanced Science Institute, RIKEN, Japan.
Abstract:
Cigarette smoke (CS) induces oxidative stress, which disables macrophage function. In this study, we examined whether Resolvin E1 (RvE1), a pro-resolving mediator known to enhance macrophage functions, attenuates the damage of macrophages by CS extract (CSE) induced oxidative stress. RvE1 blocked p47phox translocation to plasma membrane induced by CSE in a macrophage cell line, RAW264.7 cells, resulting in suppression of superoxide production. Furthermore, pretreatment of RAW264.7 cells with RvE1 restored the phagocytic activity and reduced cell death induced by treatment of CSE. These results suggest that RvE1 plays important roles in preserving macrophage function under CS-induced oxidative stress.
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