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Updated: May 10, 2026
Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
Published on: July 28, 2023
[Expression and significance of DNMT1 in human keloid fibroblast]
E Yang1, Qi-Pa Zou, Heng-Shu Zhang
1Department of Plastic and Burning Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Objective:
To investigate the expression and significance of DNA methyltransferase 1 (DNMT1) in different parts and formation time of keloid fibroblasts.
Methods:
Specimen were collected from fifty-three patients with keloid. The keloids were grouped according to formation time. Immunohistochemistry was used to assay the expression of DNMT1 in normal skin, invasive part, proliferative part and aged part. RT-PCR was adopted to assay the expression of DNMT1 mRNA in each group.
Results:
DNMT1 is mainly distributed in the nucleus of fibroblasts. The expression of DNMT1 protein and DNMTl mRNA in keloid (72%) is higher than that in normal skin fibroblasts (8%) (P = 0.001). The expression rate of DNMT1 protein and DNMT1 mRNA in shorter formation time group (100%, Score:10.47 +/- 1.85) is higher than a longer formation time group (45%,Score: 7.71 +/- 1.80) (P = 0.007). The expression rate of DNMT1 protein and DNMT1 mRNA in the invasive part of keloid (100%, Score: 10.47 +/- 1.85) is higher than the proliferative part (78%, Score: 6.63 +/- 1.75) and aged part (38%, Score: 5.53 +/- 1.55) (P = 0.001).
Conclusions:
DNMT1 plays an important role in keloid. The expression of DNMT1 tends to decrease along with the increase of the formation time in keloid. It is also related to the part of keloid. DNMT1 plays an important role in the process of keloid growth and is closely related to infiltrative growth in keloid.
