Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Inhibition of Cdk Activity

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

Inhibition of CDK Activity

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

M-Cdk Drives Transition Into Mitosis

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

M-Cdk Drives Transition Into Mitosis

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

Positive Regulator Molecules

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.

Positive Regulator Molecules

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Treatment Utilization Patterns in Cutaneous Lupus Erythematosus: A Single-Center Retrospective Analysis.

Journal of the American Academy of Dermatology·2026

Same author

RNA in situ hybridization for ISG15 and IFI6 highlights IFN-I activity in discoid lupus erythematosus.

JID innovations : skin science from molecules to population health·2026

Same author

Individualized prediction of survival after brain metastasis development in patients with breast cancer.

Journal of neuro-oncology·2026

Same author

Latexin regulates sex dimorphism in hematopoiesis via gender-specific differential expression of microRNA 98-3p and thrombospondin 1.

Cell reports·2026

Same author

Recruitment of BRD4 to the ASXL1 genomic targets depends on the extra-terminal domain of BRD4.

Nature communications·2026

Same author

ARP2/3 complex mediates the neuropathology of PTEN-deficient human neural cells downstream of mTORC1 and mTORC2 hyperactivation.

Proceedings of the National Academy of Sciences of the United States of America·2026

Search research articles

Related Experiment Video

Updated: May 10, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

The tumor suppressor CDKN3 controls mitosis.

Grzegorz Nalepa¹, Jill Barnholtz-Sloan, Rikki Enzor

¹Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

The Journal of Cell Biology

|June 19, 2013

Summary

The tumor suppressor CDKN3 is crucial for cell division (mitosis) and early cell growth. This phosphatase regulates the cell cycle by dephosphorylating CDC2, impacting cancer therapeutics.

More Related Videos

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Related Experiment Videos

Last Updated: May 10, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Area of Science:

Cell Biology
Molecular Biology
Cancer Research

Background:

Mitosis is a tightly regulated process involving intricate networks of kinases and phosphatases.
Understanding the roles of specific phosphatases in mitosis is critical for deciphering cell cycle control and identifying potential therapeutic targets.

Purpose of the Study:

To comprehensively screen human phosphatases for their roles in mitosis.
To investigate the function of the tumor suppressor CDKN3 in cell cycle regulation and mitosis.
To identify downstream targets and signaling pathways regulated by CDKN3 during mitosis.

Main Methods:

Genome-wide small interfering RNA (siRNA) screen of human phosphatases.
Cell cycle analysis and assessment of mitotic progression.
Western blotting to detect phosphorylated CDC2 (pThr-161).
Phosphokinome-wide mass spectrometry to identify CDKN3 signaling effectors.
Immunofluorescence microscopy to visualize protein localization.
Analysis of CDKN3 protein levels in brain tumor samples.

Main Results:

Identified four candidate spindle checkpoint phosphatases, including CDKN3.
Demonstrated that CDKN3 is essential for normal mitosis and the G1/S cell cycle transition.
Showed that CDKN3 dephosphorylates CDC2 at threonine-161 during mitotic exit.
Identified CKβ phosphorylated at serine 209 as a downstream target of the CDKN3-CDC2 axis, localizing to mitotic centrosomes and controlling the spindle checkpoint.
Observed down-regulation of CDKN3 protein in brain tumors.

Conclusions:

CDKN3 plays a vital role in regulating mitosis and cell cycle progression through the CDC2 signaling pathway.
The findings highlight CDKN3's function in maintaining genomic stability and its potential as a therapeutic target in cancers, particularly brain tumors.