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Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
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Published on: April 19, 2017

Lymphocyte crossmatching by flow cytometry

Robert A Bray1

  • 1Department of Pathology, Emory University Hospital, Atlanta, GA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 19, 2013
PubMed

Flow cytometry is an invaluable tool for studying lymphocyte biology. In transplantation, flow cytometry represents the most sensitive method for demonstrating the binding of HLA antibodies to cellular targets. Referred to as the flow cytometric crossmatch (FCXM), the implementation of this method has revolutionized HLA antibody assessments and facilitated increased allograft survival among highly sensitized recipients. Here we describe the methods for performing the FCXM and discuss its critical aspects, as well as the presentation of data and interpretation of results.

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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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