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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 10, 2026

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
09:04

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Published on: April 19, 2017

Lymphocyte crossmatching by flow cytometry.

Robert A Bray1

  • 1Department of Pathology, Emory University Hospital, Atlanta, GA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 19, 2013
PubMed
Summary
This summary is machine-generated.

Flow cytometry provides a sensitive method for detecting HLA antibodies in transplantation. This technique, known as the flow cytometric crossmatch (FCXM), improves allograft survival in highly sensitized patients.

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Area of Science:

  • Immunology
  • Transplantation Science
  • Cellular Biology

Background:

  • Flow cytometry is a key technique for analyzing lymphocyte populations.
  • Detecting HLA antibodies is crucial for successful organ transplantation.
  • Highly sensitized recipients face significant challenges due to pre-formed antibodies.

Purpose of the Study:

  • To detail the methodology of the flow cytometric crossmatch (FCXM).
  • To discuss critical aspects of FCXM implementation and data interpretation.
  • To highlight the impact of FCXM on assessing HLA antibodies and improving transplant outcomes.

Main Methods:

  • Description of the standard operating procedure for performing FCXM.
  • Emphasis on reagent selection and sample handling.
  • Guidelines for data acquisition and analysis in FCXM.

Main Results:

  • FCXM demonstrates high sensitivity in detecting HLA antibody binding to cellular targets.
  • Implementation of FCXM has led to a revolution in HLA antibody assessment.
  • FCXM facilitates increased allograft survival rates, particularly in challenging recipient populations.

Conclusions:

  • FCXM is an indispensable tool in transplantation for sensitive HLA antibody detection.
  • The method's standardization and interpretation are critical for clinical application.
  • FCXM significantly contributes to better outcomes for highly sensitized transplant recipients.