Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 10, 2026

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
06:29

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

Solid phase assay measuring C4d deposition to determine complement fixation by HLA-specific antibodies.

John D Smith1, Marlene L Rose

  • 1Transplant Immunology, Harefield Hospital, Imperial College, Harefield, UK.

Methods in Molecular Biology (Clifton, N.J.)
|June 19, 2013
PubMed
Summary

Detecting complement fixing HLA-specific antibodies is crucial for organ transplant success. This study presents a sensitive solid phase assay method for identifying these antibodies, aiding in recipient selection and rejection monitoring.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Space-time dependent fluid acoustics and the generation of vorticity.

The Journal of the Acoustical Society of America·2025
Same author

Oxygen uptake kinetics differentiates two from six minute walk tests in lower extremity prosthesis users.

Scientific reports·2025
Same author

Altered Storage and Function of von Willebrand Factor in Human Cardiac Microvascular Endothelial Cells Isolated from Recipient Transplant Hearts.

International journal of molecular sciences·2023
Same author

Confined acoustic line modes within a glide-symmetric waveguide.

Scientific reports·2022
Same author

Correlates of Balance and Aerobic Indices in Lower-Limb Prostheses Users on Arm Crank Exercise.

Sensors (Basel, Switzerland)·2021
Same author

Quantifying Step Count and Oxygen Consumption with Portable Technology during the 2-Min Walk Test in People with Lower Limb Amputation.

Sensors (Basel, Switzerland)·2021

Area of Science:

  • Immunology
  • Transplant Medicine

Background:

  • Human Leukocyte Antigen (HLA)-specific antibodies are critical in solid organ transplantation.
  • Accurate detection is vital for pre-transplant recipient selection and post-transplant rejection monitoring.

Purpose of the Study:

  • To describe a sensitive solid phase assay method for detecting complement fixing HLA-specific antibodies.

Main Methods:

  • Development and application of a sensitive solid phase assay.
  • Focus on identifying complement fixing HLA-specific antibodies.

Main Results:

  • The described method enables sensitive detection of complement fixing HLA-specific antibodies.
  • This assay complements existing methods for antibody detection.

More Related Videos

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion
06:54

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

Published on: June 15, 2019

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
08:26

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum

Published on: March 29, 2010

Related Experiment Videos

Last Updated: May 10, 2026

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
06:29

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion
06:54

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

Published on: June 15, 2019

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
08:26

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum

Published on: March 29, 2010

Conclusions:

  • The presented solid phase assay is a valuable tool for identifying complement fixing HLA-specific antibodies.
  • This method can improve patient management in solid organ transplantation.