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Related Experiment Video

Updated: May 10, 2026

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
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Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes

Published on: January 7, 2013

Cylex ImmuKnow Cell Function Assay.

Adriana Zeevi1, John Lunz

  • 1University of Pittsburgh Medical Center, Pittsburgh, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 19, 2013
PubMed
Summary

Monitoring immunosuppression levels alone is insufficient in solid organ transplant recipients. The ImmuKnow Immune Cell Function Assay measures T cell activity to assess immune competence, aiding in personalized immunosuppression strategies.

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Area of Science:

  • Immunology
  • Transplantation Medicine
  • Clinical Diagnostics

Background:

  • Immunosuppression is crucial for solid organ transplantation but carries risks of morbidity and mortality.
  • Current monitoring of trough immunosuppression levels is inadequate for assessing immune response suppression.
  • Assessing immune competence is vital for managing transplant recipients.

Purpose of the Study:

  • To evaluate the ImmuKnow Immune Cell Function Assay as a marker of global immune competence in transplant recipients.
  • To correlate T cell activation changes with the risks of transplant rejection and infection.
  • To determine the utility of the ImmuKnow test in identifying immune-compromised patients and guiding immunosuppression therapy.

Main Methods:

  • The ImmuKnow Immune Cell Function Assay was utilized to measure CD4+ T cell activity.
  • Changes in T cell activation were analyzed.
  • The assay's performance was assessed in conjunction with other clinical parameters.

Main Results:

  • The ImmuKnow test measures CD4+ T cell activity as a marker of immune competence.
  • T cell activation levels were correlated with the relative risks of rejection and infection.
  • The assay demonstrated utility in identifying immune-compromised patients.

Conclusions:

  • Monitoring trough immunosuppression levels alone is insufficient for transplant recipients.
  • The ImmuKnow Immune Cell Function Assay provides a measure of immune competence.
  • This assay, combined with clinical data, aids in tailoring individualized immunosuppression regimens to reduce risks.

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