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Updated: May 10, 2026

09:11
Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
Published on: July 16, 2019
Detection of intracellular cytokines
Nancy L Reinsmoen1, Chih-Hung Lai
1HLA Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 19, 2013
Abstract:
The intracellular cytokine method allows for a multiparametric readout by flow cytometry with precise phenotyping of the responding T cells. The intracellular cytokine staining of cells that have been fixed and permeabilized following a short-term activation and staining with antibodies to cell surface markers allows for identification of the cellular origin of the cytokine accumulated product.

