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Published on: April 23, 2019
Development of a LC-ESI-MS/MS approach for the rapid quantification of main wine organic acids in human urine
Jorge Regueiro1, Anna Vallverdú-Queralt, Jesús Simal-Gándara
1Nutrition and Bromatology Group, Analytical and Food Chemistry Department, Faculty of Food Science and Technology, Ourense Campus, University of Vigo , Ourense 32004, Spain.
Abstract:
The analysis of food components and their metabolome in urine has recently found a growing interest due their potential ability to reflect specific dietary intakes. In the present work, a fast, simple, and environmentally friendly method based on liquid chromatography coupled to electrospray ionization tandem mass spectrometry was developed for the analysis of main wine organic acids in human urine. The proposed method was evaluated in terms of linearity, precision, accuracy, and limits of detection. Quantitative recovery (96-102%) and satisfactory interday precision (RSD <6%) were achieved for all target compounds. To demonstrate the applicability of the method, urine samples from five male volunteers were analyzed before and after consumption of a single moderate dose (200 mL) of red wine. A significant increase (p < 0.01) in the urinary concentration of tartaric and malic acids was observed.
