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Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
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Single embryo-coupled gate field effect transistor for elective single embryo transfer.

Toshiya Sakata1, Akiko Saito, Jinji Mizuno

  • 1Department of Materials Engineering, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan. sakata@biofet.t.u-tokyo.ac.jp

Analytical Chemistry
|June 20, 2013
PubMed
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AMH Concentration and Gene Expression Level in Each Follicle and Their Relationship With Fertilization and Blastocyst Formation in Intracytoplasmic Sperm Injection.

Reproductive medicine and biology·2025

Researchers developed a novel field-effect transistor (FET) biosensor to noninvasively monitor single mouse embryo activity after in vitro fertilization (IVF). This device provides objective electrical signals, aiding in embryo evaluation and promoting elective single embryo transfer (eSET).

Area of Science:

  • Biomedical Engineering
  • Reproductive Technology
  • Biosensor Technology

Background:

  • Assessing embryo viability after in vitro fertilization (IVF) is crucial for successful assisted reproductive technology (ART).
  • Current methods often rely on subjective morphological evaluation, lacking quantitative and continuous monitoring capabilities.
  • Objective assessment of early embryo development is needed to improve success rates and support practices like elective single embryo transfer (eSET).

Purpose of the Study:

  • To develop and experimentally demonstrate a novel semiconductor-based field-effect transistor (FET) biosensor for noninvasive, quantitative, and continuous monitoring of single mouse embryo activity.
  • To establish an objective electrical signal readout for evaluating embryo viability, complementing subjective morphological assessments.
  • To provide a technological platform that can advance ART by enabling more informed embryo selection.

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Main Methods:

  • Fabrication and utilization of a field-effect transistor (FET) biosensor.
  • Detection of changes in hydrogen ion concentration, indicative of dissolved carbon dioxide from cellular respiration.
  • Continuous electrical signal recording correlated with embryo metabolic activity during cleavage stages.
  • Experimental validation using single mouse embryos post-IVF.

Main Results:

  • The FET biosensor successfully detected noninvasive, quantitative, and continuous changes in embryo activity.
  • Detected hydrogen ion fluctuations, induced by dissolved carbon dioxide from cellular respiration, provided a reliable indicator of embryo metabolic status.
  • The electrical signals generated by the FET biosensor served as an objective measure of embryo activity, distinct from subjective morphological observations.

Conclusions:

  • The developed FET biosensor offers a novel and effective method for objective, real-time monitoring of single embryo activity post-IVF.
  • This technology has the potential to significantly improve the accuracy of embryo assessment in ART.
  • The platform supports the advancement of eSET by providing reliable data for embryo selection, ultimately aiming to enhance ART success rates.