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Long-term nitric oxide release and elevated temperature stability with S-nitroso-N-acetylpenicillamine (SNAP)-doped
Elizabeth J Brisbois1, Hitesh Handa, Terry C Major
1Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
Biomaterials
|June 20, 2013
Summary
This study developed a novel S-nitroso-N-acetylpenicillamine (SNAP) doped polymer coating for medical devices. The coating effectively releases nitric oxide (NO), significantly improving blood compatibility and reducing clot formation in extracorporeal circulation circuits.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Medical Device Engineering
Background:
- Nitric oxide (NO) is crucial for preventing thrombosis by inhibiting platelet activation and adhesion.
- Endothelial cells produce NO, maintaining blood vessel health and preventing clot formation.
- Biomaterials with controlled NO release mimic natural anti-thrombotic mechanisms.
Purpose of the Study:
- To investigate polymers doped with S-nitroso-N-acetylpenicillamine (SNAP) for controlled NO release.
- To evaluate the anti-thrombotic properties of a SNAP-doped Elast-eon E2As polymer coating.
- To assess the stability and efficacy of the SNAP/E2As coating in blood-contacting applications.
Main Methods:
- Five biomedical polymers were doped with SNAP to control NO and SNAP release.
- SNAP was incorporated into Elast-eon E2As for a homogeneous, stable coating.
- Coated extracorporeal circulation (ECC) circuits were tested in a rabbit model for 4 hours.
Main Results:
- The SNAP/E2As coating demonstrated excellent storage stability, retaining 82% SNAP after 2 months at 37°C.
- Platelet count was preserved at 100 ± 7% in SNAP/E2As coated circuits versus 60 ± 6% in controls.
- Thrombus area was reduced in SNAP/E2As coated circuits (2.3 pixels/cm²) compared to controls (3.4 pixels/cm²).
Conclusions:
- The SNAP/E2As coating offers a promising strategy for enhancing the thromboresistance of medical devices.
- Controlled NO release from the SNAP/E2As polymer significantly improves hemocompatibility.
- This material exhibits superior storage stability compared to existing NO-releasing polymers.
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