Related Experiment Video
Updated: May 10, 2026
Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome
Published on: March 2, 2018
Can the L5178Y Tk+/- mouse lymphoma assay detect epigenetic silencing?
Tsu-Fan Cheng1, Geoffrey W Patton, Kristi Muldoon-Jacobs
1US Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Food Additive Safety, Division of Food Contact Notification, College Park, MD 20740, USA.
Abstract:
The mouse lymphoma L5178Y Tk(+/-) assay is broadly used in toxicology to assess genotoxicity because of its known sensitivity to genotoxicants that act through a variety of mechanisms, which may include epigenetic DNA methylation. This brief article highlights the studies that have contributed to this conjecture and suggests an addition to the experimental design that could identify if the test substance is a potential epimutagen acting via hypermethylation.
More Related Videos
13:21Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
Published on: June 16, 2017
09:52A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
Published on: December 4, 2018