Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sections from double mesas obtained at the same tissue plane.

G Ellender1

  • 1School of Dental Science, University of Melbourne, Parkville, Australia.

Stain Technology
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oral Health Impacts on Flavor and Significance in Dental Treatment.

JDR clinical and translational research·2021
Same author

Growth and bone remodelling in a scorbutic rat model.

Australian dental journal·1996
Same author

Cytotoxicity of gallium and indium ions compared with mercuric ion.

Journal of dental research·1994
Same author

Sealing ability of three materials used to repair lateral root perforations.

Journal of endodontics·1993
Same author

Osteogenic capacity of collagen in repair of established periodontal defects.

Clinical materials·1991
Same author

The biocompatibility testing of some dental amalgams in vivo.

Australian dental journal·1990
Same journal

Further observations on ethyl alcohol substitutes.

Stain technology·2010
Same journal

Sterile milk for background in capsule staining.

Stain technology·2010
Same journal

The use of clarite in preparing whole microscopic mounts.

Stain technology·2010
Same journal

A simplified method of preparation of di-ammine-silver hydroxide for reticulum impregnation; comments on the nature of the so-called sensitization before impregnation.

Stain technology·2010
Same journal

A simplified stain for hemoglobin in tissues or smears using patent blue.

Stain technology·2010
Same journal

Improved fixation in vitally stained methylene blue preparations.

Stain technology·2010
See all related articles

This study introduces a novel tissue sectioning method for ultrathin sections. It enables comparative morphometric studies by precisely sectioning widely separated tissue areas.

Area of Science:

  • Microscopy and Histology
  • Materials Science
  • Biological Sciences

Background:

  • Obtaining comparable ultrathin sections from distant tissue regions is challenging.
  • Current methods limit the analysis of spatially separated areas within a single specimen.
  • Comparative morphometric studies require precise sectioning of multiple tissue sites.

Purpose of the Study:

  • To develop a method for obtaining ultrathin sections from two distinct, horizontally or vertically separated areas of a tissue block.
  • To facilitate comparative morphometric studies by enabling analysis of widely separated tissue regions within the same specimen.
  • To overcome limitations in current sectioning techniques for spatially distant areas of interest.

Main Methods:

  • Preparation of two horizontally separated mesas on a tissue block.

Related Experiment Videos

  • Sectioning the first mesa with a knife rotated 2-3 degrees around its vertical axis.
  • Sectioning the second mesa with the knife rotated 4-6 degrees in the opposite direction.
  • Adjusting the vertical inclination of the block to cut two additional vertically separated mesas.

    • Main Results:

    • Successful acquisition of ultrathin sections from horizontally and vertically separated tissue areas.
    • The described method allows for virtually identical planes of sectioning across separated mesas.
    • Demonstrated feasibility for comparative morphometric analysis of disparate tissue regions.

    Conclusions:

    • The novel sectioning technique is valuable for comparative morphometric studies.
    • It enables precise analysis of material from opposite sides of individual specimens.
    • This method expands the possibilities for detailed histological and morphological investigations.