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Preparation of Homogeneous MALDI Samples for Quantitative Applications
Published on: October 28, 2016
Introduction to hyphenated techniques and their applications in pharmacy
Kalpesh N Patel1, Jayvadan K Patel, Manish P Patel
1Nootan Pharmacy College, Kamana Crossing, Visnagar - 384 315, India.
Pharmaceutical Methods
|June 20, 2013
Summary
Hyphenated analytical techniques, combining separation and spectroscopic detection, offer powerful tools for natural product analysis. These advanced methods are crucial for studying biomaterials, herbal products, and metabolomics.
Area of Science:
- Analytical Chemistry
- Natural Product Chemistry
Background:
- Hyphenated techniques couple separation methods with on-line spectroscopic detection.
- Recent advancements have expanded their use in biomaterial analysis, particularly for natural products.
Purpose of the Study:
- To review recent advances in hyphenated techniques for natural product analysis.
- To highlight applications in various stages of natural product research and quality control.
Main Methods:
- Gas Chromatography-Mass Spectrometry (GC-MS)
- Liquid Chromatography-Mass Spectrometry (LC-MS)
- Liquid Chromatography-Fourier Transform Infrared Spectroscopy (LC-FTIR)
- Liquid Chromatography-Nuclear Magnetic Resonance (LC-NMR)
- Capillary Electrophoresis-Mass Spectrometry (CE-MS)
Main Results:
- Demonstrated broad applicability in pre-isolation analysis, isolation, and on-line detection of natural products.
- Showcased utility in chemotaxonomic studies, chemical fingerprinting, and quality control of herbal products.
- Highlighted effectiveness in natural product dereplication and metabolomic studies.
Conclusions:
- Hyphenated techniques are indispensable for comprehensive natural product analysis.
- These methods significantly advance research in natural products, herbal medicine, and metabolomics.
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