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A validated method for development of atovaquone as API and tablet dosage forms by UV spectroscopy
Kalpesh N Patel1, Jayvadan K Patel, Manish P Patel
1Nootan Pharmacy College, Kamana Crossing, Visnagar - 384 315, India.
Pharmaceutical Methods
|June 20, 2013
Summary
A new spectrophotometric method accurately quantifies Atovaquone in bulk and tablet forms. This validated method offers a simple, sensitive, and reproducible approach for routine Atovaquone analysis.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- Accurate quantification of Atovaquone is crucial for pharmaceutical quality control.
- Existing analytical methods may require improvement in terms of simplicity and efficiency.
Purpose of the Study:
- To develop and validate a simple spectrophotometric method for Atovaquone estimation.
- To assess the method's applicability for bulk drug and tablet dosage forms.
Main Methods:
- Spectrophotometric analysis of Atovaquone in methanol at 251 nm.
- Validation using Beer's law, statistical analysis, and recovery studies.
- Application to marketed tablet formulations.
Main Results:
- Beer's law adherence in the 1-10 μg/mL range (slope=0.111, intercept=0.012).
- Accurate quantification in tablet formulation (99.14 ± 0.66% label claim).
- High precision and accuracy demonstrated through recovery studies (100.09 ± 1.14%).
Conclusions:
- The developed spectrophotometric method is simple, sensitive, and reproducible.
- It is suitable for routine quality control analysis of Atovaquone in bulk and pharmaceutical products.
- This method provides a reliable tool for ensuring drug quality and efficacy.
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