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RP-UPLC method development and validation for the simultaneous estimation of ibuprofen and famotidine in
Yarram Ramakoti Reddy1, Kakumani Kishore Kumar, Mrp Reddy
1Department of Chemical Science and Technology, Institute of science and Technology, Jawaharlal Nehru Technological University, Hyderabad, India.
Aim And Background:
A stability-indicating LC method was developed for the simultaneous determination of Ibuprofen and Famotidine in pharmaceutical dosage forms.
Materials And Methods:
The chromatographic separation was achieved on Acquity UPLC BEH C-18,50 mm × 2.1 mm and 1.7 μm column with gradient elution. The mobile phase A contains a mixture of 50 mM sodium acetate buffer (pH 5.5): methanol (85:15, v/v), and the mobile phase B contains a mixture of 50 mM sodium acetate buffer (pH 5.5): methanol (25:75, v/v). The flow rate was 0.3 mL min(-1), and the detection wavelength was 260 nm.
Results:
The limit of detection for Ibuprofen and Famotidine was 1.6 and 1.2 μg mL(-1), respectively. The limit of quantification (LOQ) for Ibuprofen and Famotidine was 5.1 and 4.3 μg mL(-1), respectively.
Conclusion:
This method was validated for accuracy, precision, and linearity. The method was also found to be stability indicating.
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