Related Experiment Video
Updated: May 10, 2026
09:04
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
Microarray gene expression profiling of neural tissues in bovine spastic paresis
Lorraine Pariset1, Silvia Bongiorni, Susana Bueno
1Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), University of Tuscia, via S, Camillo de Lellis snc, Viterbo, 01100, Italy. alessio@unitus.it
BMC Veterinary Research
|June 21, 2013
Summary
Bovine Spastic Paresis (BSP) is a genetic neuromuscular disorder in cattle. This study used cDNA microarrays to identify affected gene pathways, revealing potential links to neurodegeneration and synaptic transmission defects.
Area of Science:
- Genomics
- Molecular Biology
- Veterinary Science
Background:
- Bovine Spastic Paresis (BSP) is a neuromuscular disorder in cattle, characterized by spasticity and reduced weight gain.
- The genetic basis of BSP is suspected to be autosomal recessive with incomplete penetrance, but causative genes remain unidentified.
- Understanding the molecular pathways affected by BSP is crucial for disease investigation.
Purpose of the Study:
- To identify metabolic pathways and genes affected in Romagnola cattle with Bovine Spastic Paresis (BSP) using cDNA microarrays.
- To investigate the genome-level alterations associated with BSP.
- To gain insights into the molecular mechanisms underlying this poorly understood genetic disease.
Main Methods:
- cDNA microarray analysis was performed on control and BSP-affected Romagnola cattle.
- Differentially expressed genes were identified and subjected to KEGG pathway functional clustering.
- Chromosome distribution of differentially expressed genes was analyzed to identify potential disease-harboring genomic locations.
Main Results:
- Microarray analysis identified 268 differentially expressed genes between control and affected individuals.
- Affected genes were predominantly involved in Cell Communication, Signal Transduction, and Nervous System pathways.
- A significant enrichment of pathways related to Neurodegenerative Diseases was observed among under-expressed genes in affected samples.
Conclusions:
- cDNA microarrays provided valuable insights into BSP, analyzing approximately 15,000 transcripts.
- Data suggest defective glycinergic synaptic transmission and altered calcium signaling proteins in BSP development.
- This study contributes novel molecular information on BSP, potentially aiding future research and offering insights into human diseases.
